Kay Francis
— Josephine "Jo" Bhaer
Jack Oakie
— William "Willie" Deming
George Bancroft
— Major I.I. Burdle
Jimmy Lydon
— Daniel "Danny" Burdle
Ann Gillis
— Nan
Carl Esmond
— Professor Bhaer
Richard Nichols
— Teddy Bhaer
Casey Johnson
— Robby
Francesca Santoro
— Bess
Johnny Burke
— Silas
Lillian Randolph
— Asia
Sammy McKim
— Tommy
Edward Rice
— Demi
Anne Howard
— Daisy
Jimmy Zahner
— Jack
Bobby Cooper
— Adolphus
Schuyler Standish
— Nat
Paul Matthews
— Stuffy
Tony Neil
— Ned
Fred Estes
— Emmett
Douglas Rucker
— Billy
Donald Rackerby
— Frank
William Demarest
— Constable Tom Thorpe
Sterling Holloway
— Reporter
Isabel Jewell
— Stella
Charles Arnt
— Drunk in Medicine Show
Stanley Blystone
— Bartender
Nora Cecil
— Head of Orphanage
Hal K. Dawson
— Telegraph Operator
Sarah Edwards
— Landlady