Kazuyuki Okitsu
— Jonathan Joestar (voice)
塩屋翼
— Will Anthonio Zeppeli (voice)
Yoji Ueda
— Robert E.O. Speedwagon (voice)
Ayako Kawasumi
— Erina Pendleton (voice)
Masashi Sugawara
— George Joestar I (voice)
Yumiko Kobayashi
— Poco (voice)
大木民夫
— Tonpetty (voice)
武虎
— Dire (voice)
Nobuo Tobita
— Straizo (voice)
Takehito Koyasu
— Dio Brando (voice)
中博史
— Wang Chan (voice)
楠見尚己
— Jack the Ripper (voice)
Kenjirō Tsuda
— Bruford (voice)
Tetsu Inada
— Tarkus (voice)
宮澤正
— Dario Brando (voice)
Endo Aya
— Poco's Sister (voice)
加藤将之
— Father Styx (voice)
Tōru Ōkawa
— Narrator (voice)