Nova Pilbeam
— Lady Jane Grey
Cedric Hardwicke
— Earl of Warwick
John Mills
— Lord Guilford Dudley
Felix Aylmer
— Edward Seymour
Leslie Perrins
— Thomas Seymour
Frank Cellier
— Henry VIII
Desmond Tester
— Edward VI
Gwen Ffrangcon Davies
— Mary Tudor (as Gwen Ffrangcon-Davies)
Martita Hunt
— Jane's Mother
Miles Malleson
— Jane's Father
Sybil Thorndike
— Ellen
Peter Croft
— Confidant of Thomas Seymour
Albert Davies
— Barnaby Fitzpatrick
Shaun Desmond
— Undetermined Role
Edward Dignon
— Undetermined Role
Roy Emerton
— Squire
C. V. France
— Clergy at Execution
Arthur Goullet
— Sir John Gates
Henry Hallett
— Undetermined Role
John Laurie
— John Knox
Eugene Leahy
— Undetermined Role
Fewlass Llewellyn
— Undetermined Role
Cicely Paget-Bowman
— Courtier
John Singer
— Boy
John Turnbull
— Arundel