Paul Reubens
— Pee-wee Herman
Joe Manganiello
— Joe Manganiello
Jessica Pohly
— Pepper
Alia Shawkat
— Bella
Stephanie Beatriz
— Freckles
Jordan Black
— Yul / Party Coordinator (voice)
Doug Cox
— Mr. Murgatroyd
Linda Porter
— Mrs. Rose
Monica Horan
— Ruby the Crossing Guard
Brian Palermo
— Marvin
Katherine Vanderlinden
— Emily
Tara Buck
— Beverly
John H. Mayer
— Pharmacist
Richard Riehle
— Dan
Dave Power
— Dudley
Josh Meyers
— Sylvester
Corey Martin Craig
— Clovis
Paul Rust
— Ernie
Robert R. Shafer
— Construction Worker
John Moody
— Construction Worker
Daniel Lue
— Police Officer
Darrin Henson
— Police Officer
Clayton Cannon
— Police Officer
Patrick Egan
— Gordon the Traveling Salesman
Gloria Vassy
— Panicked Female Passenger
Erick Chavarria
— Panicked Male Passenger
Frank Collison
— Clay
Lynne Marie Stewart
— Jimmy
Karen Maruyama
— Tourist at Snake Farm
Hal Landon Jr.
— Farmer Brown