Mina Blum
— Kate Pinkerton
Carlo Bosi
— Goro, a matchmaker
Fiorenza Cedolins
— Cio-Cio-san (Madama Butterfly)
Francesco Franci
— Suzuki, her maid
Marcello Giordani
— B. F. Pinkerton, Lieutenant in the United States Navy
Juan Pons
— Sharpless, United States consul at Nagasaki
Carlo Striuli
— The Bonze, Cio-Cio-san's uncle
Alessandro Battiato
— Prince Yamadori
Angelo Nardinocchi
— The Imperial Commissioner
Giovanni Scordino
— The Official Registrar
Veronica Simeoni
— Cio-Cio-san's mother
Maria Letizia Grosselli
— The cousin