Rhett Akins
— Tom Sawyer (voice)
Mark Wills
— Huckleberry Finn (voice)
Hynden Walch
— Becky Thatcher (voice)
Lee Ann Womack
— Becky Thatcher (singing voice)
Clea Lewis
— Amy Lawrence (voice)
Alecia Elliott
— Amy Lawrence (singing voice)
Betty White
— Aunt Polly (voice)
Dean Haglund
— Sid (voice)
Richard Kind
— Mr. Dobbins (voice)
Kevin Michael Richardson
— Injurin' Joe (voice)
Hank Williams, Jr.
— Injun' Joe (singing voice)
Don Knotts
— Mutt Potter (voice)
Waylon Jennings
— Judge Thatcher (voice)
Dee Bradley Baker
— Rebel
Pat Corley
— Sheriff McGee (voice)
Marty Stuart
— Reverend (voice)
Thom Adcox-Hernandez
— Deputy Bean (voice)
Sheryl Bernstein
— (voice)
Jennifer Hale
— (voice)
David Kaufman
— (voice)