Gary Cooper
— Wild Bill Hickok
Jean Arthur
— Calamity Jane
James Ellison
— William "Buffalo Bill" Cody
Charles Bickford
— John Lattimer
Helen Burgess
— Louisa Cody
Porter Hall
— Jack McCall
Paul Harvey
— Yellow Hand
Victor Varconi
— Painted Horse
John Miljan
— General George A. Custer
Frank McGlynn Sr.
— Abraham Lincoln
Granville Bates
— Van Ellyn
Anthony Quinn
— Cheyenne Indian
George 'Gabby' Hayes
— Breezy
Mark Strong
— Wells Fargo Agent (uncredited)
Frank Albertson
— A Young Trooper
Purnell Pratt
— Capt. Wood
Fred Kohler
— Jake - A Teamster
Pat Moriarity
— Sgt. McGinnis
Charles Judels
— Tony - The Barber
Harry Woods
— Quartermaster Sergeant
Francis McDonald
— A River Gambler
George Ernest
— A Boy
George MacQuarrie
— Gen. Merritt
Fuzzy Knight
— Dave
Everett Brown
Curtis Nero
— Roustabout