Magda Vášáryová
— Mary Stuart
Joan Sutherland
— Mary Stuart (singing voice)
Kamila Magálová
— Queen Elizabeth I
Huguette Tourangeau
— Queen Elizabeth I (singing voice)
Milan Kňažko
— Earl of Leicester
Luciano Pavarotti
— Earl of Leicester (singing voice)
Přemysl Kočí
— George Talbot
Roger Soyer
— George Talbot (singing voice)
Jan Přeučil
— William Cecil
James Morris
— William Cecil (singing voice)
Jiřina Jirásková
— Hanna Kennedy
Margreta Elkins
— Hanna Kennedy (singing voice)
Luděk Munzar
— Amias Paulet
Vladimír Dlouhý
— Mortimer
Karel Roden
— William Davison
Jiří Vala
— Melville
Zdeněk Ornest
— Kent (uncredited)
Kornelia Boje
— Mary Stuart (voice)
Christa Berndl
— Queen Elizabeth I (voice)
Randolf Kronberg
— Earl of Leicester (voice)
Bettina Schön
— Hanna Kennedy (voice)
Hartmut Reck
— Amias Paulet (voice)
Martin Umbach
— Mortimer (voice)
Michael Cramer
— Melville (voice)