In Space with Markiplier

Mark Fischbach

— Mark

Jordan Bradley

— Captain

Lio Tipton

— Lady

Pamela Horton

— Celcionna F. Kelvina

Mick Lauer

— Gunther B. Gunnerson

Robert Muyskens

— Burt

Steve Taylor

— Computer (voice)

Matthew Patrick

— Theory Crew

Wade Barnes

— Wug

Morgan Calhoun

— Bandit

Arin Hanson

— Narrator (voice)

Holt Boggs

— Shuttle Pilot

Ethan Nestor

— Wormhole

Baldermort

— Invincible Tower

Tyler Scheid

— First Mate

Linda Bradshaw

— Dorene L. Whitacre

Brian Wecht

— Ninja Crew

Dan Avidan

— Sexy Crew

Imane Anys

— Cryo Computer (voice)

Seán McLoughlin

— Drones

Carlos Pena

— Crew 1

Roderick Loving

— Crew 2

Jason C Campbell

— Crew 3

Judy Lee

— Crew 4

Sophia Tinsley

— Crew 5

Reggie Lussier

— Crew 6

Kai Woodley

— Crew 7

Gabriel Leite

— Crew 8

Amani Alexander

— Crew 9

Adam Weightman

— Crew 10