Tara Strong
— Batgirl / Harleen Quinzel (voice)
Nicole Sullivan
— Supergirl (voice)
Grey DeLisle
— Wonder Woman (voice)
Myrna Velasco
— Jessica Cruz (voice)
Kimberly Brooks
— Bumblebee / Eliza Danvers / Alexandra (voice)
Kari Wahlgren
— Zatanna / Lillian Luthor / News Anchor (voice)
Keith Ferguson
— Jeremiah Danvers / Demo-Bot Foreman (voice)
Cassandra Lee Morris
— Lena Luthor (voice)
Will Friedle
— Lex Luthor / Truant Officer / Aiden (voice)
Phil LaMarr
— Mr. Zatara / Barry Allen (voice)
Jason Spisak
— Hal Jordan (voice)
Cree Summer
— Hippolyta (voice)
Fred Tatasciore
— Commissioner Gordon / Mr. Chapin (voice)